Hydrazide Gel

Description:

 

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Hydrazide Gel is a high efficiency glycoprotein binding medium, which will bind oxidised sugar molecules in the glycosyl groups of Anti-bodies and does not bind to amino acids.This important fuction makes it specific to immunoglobulins where the gycosylated area is distinctly located away from the hper-variable binding region. By using Hydrazide gel to exploit this antibodies may be linked to a solid phase support via the oxidised glycosyl groups.Thus preserving the antibody integrity,which no other linking method can guarantee. The method employs the oxidation of sugar molecules with sodium meta-periodate which converts vicinal pairs of hydroxyl groups to aldehydes, (-CHO),it is these which bind hydrazide agarose.

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