20X SSC – Bacterial Filtered

Description:

Molecular Biology hybridisation transfer buffer  saline-sodium citrate (SSC) buffer is used to control stringency for washing steps in protocols for  Southern Blotting, Northern Blotting, in situ hybridisation DNA microarrays. 20X SSC may be used to prevent drying of Agarose and PAGE gels during a vacuum transfer.

A 20X stock solution consists

3M Sodium Chloride

300mM Tris-Sodium Citrate

pH7.0

DNase/RNase non detected.